Robo Koopa - S1-E52
Continuity mistake: When the Marios are hurting enemies and freeing Dr. Nerdnik, Mario kicks through a gate at the start of the scene in order to get inside. This gate disappears, once when Bunsun pushes the doctor through the hole, and twice at the end of the scene. (00:04:45 - 00:06:30)
Rolling Down the River - S1-E5
Continuity mistake: When the Koopa Troopas discover that Mario and Luigi cheated, Mario's gloves disappear. (00:05:29)
Rolling Down the River - S1-E5
Continuity mistake: In one shot of Toad being thrown into the dungeon, his blue trousers turn white. (00:04:48)
Rolling Down the River - S1-E5
Continuity mistake: When King Koopa says that he wants to capture the Mario Bros, part of his underbelly changes from yellow to green. (00:03:40)
Revealing mistake: In many episodes, when the outside of Mario and Luigi's business is shown, the edge of the backdrop can be seen.
Other mistake: In many of the live-action segments, the Alarmtron logo on the alarm in the Mario Bros' residence has been partially covered to read "Acarmtron" instead. In addition, in several episodes, the phone number for Alarmtron is also partially covered.
Revealing mistake: When the crocodiles approach the tree, one of them is layered under a rock. (00:11:57)
Revealing mistake: In the live-action segment, when the Super Plant chases Mario and Luigi, the actor's shoes can be seen. This also happens when the Super Plant heads over to Dr. Toby. (00:17:43 - 00:18:57)
Robo Koopa - S1-E52
Continuity mistake: When Robo Koopa destroys a building, the black border around his chestplate turns white, like the chestplate. This also occurs when he says that the Mario Bros. cannot help Princess Toadstool and Toad. (00:03:22 - 00:08:34)
Robo Koopa - S1-E52
Revealing mistake: When Robo Koopa bursts out of the workshop, he is briefly layered over a wall. (00:08:49)
Star Koopa - S1-E51
Revealing mistake: When Princess Toadstool zaps the Stormtroopa holding Luigi, the light around it is misplaced. (00:06:48)
Robo Koopa - S1-E52
Revealing mistake: When Mario and his friends run, as they fight off Koopa's minions, Luigi runs in midair. (00:07:12)
Star Koopa - S1-E51
Continuity mistake: When Darth Koopa freezes Mario, his Freeze Gun is red, but when he freezes his friends, it turns grey. (00:08:21)
Star Koopa - S1-E51
Continuity mistake: When Mario asks if his friends have their seatbelts on, Princess Toadstool's brooch changes from yellow with a blue centre to blue with a yellow centre. (00:12:01)
Star Koopa - S1-E51
Revealing mistake: When Princess Toadstool explains that the Mushroom Colonists have a right to be on their planet, a line on her dress appears over her right arm. (00:07:13)
Continuity mistake: Throughout most of the episode, the mayor's mustache is brown, but in his initial appearance, it is black instead. (00:04:34)
Star Koopa - S1-E51
Continuity mistake: After Darth Koopa tells Mouser to speed up the Koopa Star, his costume disappears. (00:04:58)
Continuity mistake: When the mayor points to the barbecue, the green linings on his shirt turn skin colour. (00:09:48)
Continuity mistake: When the mayor says that he and his friends should head for higher ground, the red spots on Toad's cap turn purple, like his shoes. (00:11:29)
Continuity mistake: When Princess Toadstool says that one of the crocodiles wants to eat Toad, her left eyebrow disappears briefly. (00:05:18)