Other mistake: During "My Freeze Ray", there's a shot where Billy, Penny, and several other people open doors to the laundry machines in sequence. When Billy opens the door to his machine, you can see the reflection of everyone else just standing there, waiting for their cue to walk forward.
Continuity mistake: During the very beginning of "My Freeze Ray", as Dr. Horrible is about to set down his basket of laundry, the bottle of detergent sitting on top of his clothing rotates 90 degrees after the scene briefly cuts to Penny.
Dr. Horrible: Oh goodness, look at my wrist, I have to go.
Trivia: After the series became a major success upon release in 2008, director Joss Whedon teased that he would be doing a sort-of "sequel" at some point in the future. At one point, an outline and a number of songs for the sequel were written, though due to other obligations, Whedon had to push back production. As of 2018, the long-rumored sequel has yet to materialize, though everyone involved has continued to hint that a follow-up may still happen eventually.
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